Structural characterization of the N‐terminal part of the MERS‐CoV nucleocapsid by X‐ray diffraction and small‐angle X‐ray scattering
Identifieur interne : 001438 ( Main/Exploration ); précédent : 001437; suivant : 001439Structural characterization of the N‐terminal part of the MERS‐CoV nucleocapsid by X‐ray diffraction and small‐angle X‐ray scattering
Auteurs : Nicolas Papageorgiou [France] ; Julie Lichière [France] ; Amal Baklouti [France] ; François Ferron [France] ; Marion Sévajol [France] ; Bruno Canard [France] ; Bruno Coutard [France]Source :
- Acta Crystallographica Section D [ 2059-7983 ] ; 2016-02-01.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Nucleocapsid Proteins.
- chemistry : Middle East Respiratory Syndrome Coronavirus.
- Crystallization, Crystallography, X-Ray, Models, Molecular, Protein Multimerization, Protein Structure, Tertiary, Scattering, Small Angle.
Abstract
The N protein of coronaviruses is a multifunctional protein that is organized into several domains. The N‐terminal part is composed of an intrinsically disordered region (IDR) followed by a structured domain called the N‐terminal domain (NTD). In this study, the structure determination of the N‐terminal region of the MERS‐CoV N protein via X‐ray diffraction measurements is reported at a resolution of 2.4 Å. Since the first 30 amino acids were not resolved by X‐ray diffraction, the structural study was completed by a SAXS experiment to propose a structural model including the IDR. This model presents the N‐terminal region of the MERS‐CoV as a monomer that displays structural features in common with other coronavirus NTDs.
The structural characterization of the N‐terminal part of the nucleocapsid from Middle East respiratory syndrome coronavirus (MERS‐CoV), a recently emerging virus, is reported. The structure of the N‐terminal region, which includes a disordered tail followed by a globular domain, was obtained by combining X‐ray diffraction and SAXS.
Url:
- https://api.istex.fr/ark:/67375/WNG-B1GHL0MQ-1/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7159594
DOI: 10.1107/S2059798315024328
Affiliations:
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wicri:rule="url">France</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Acta Crystallographica Section D</title><title level="j" type="alt">ACTA CRYSTALLOGRAPHICA SECTION D</title><idno type="ISSN">2059-7983</idno><idno type="eISSN">2059-7983</idno><imprint><biblScope unit="vol">72</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="192">192</biblScope><biblScope unit="page" to="202">202</biblScope><biblScope unit="page-count">10</biblScope><publisher>International Union of Crystallography</publisher><pubPlace>5 Abbey Square, Chester, Cheshire CH1 2HU, England</pubPlace><date type="published" when="2016-02-01">2016-02-01</date></imprint><idno type="ISSN">2059-7983</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">2059-7983</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term><term>Models, Molecular</term><term>Nucleocapsid Proteins (chemistry)</term><term>Protein Multimerization</term><term>Protein Structure, Tertiary</term><term>Scattering, Small Angle</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Diffusion aux petits angles</term><term>Modèles moléculaires</term><term>Multimérisation de protéines</term><term>Protéines nucléocapside ()</term><term>Structure tertiaire des protéines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Models, Molecular</term><term>Protein Multimerization</term><term>Protein Structure, Tertiary</term><term>Scattering, Small Angle</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Diffusion aux petits angles</term><term>Modèles moléculaires</term><term>Multimérisation de protéines</term><term>Protéines nucléocapside</term><term>Structure tertiaire des protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">The N protein of coronaviruses is a multifunctional protein that is organized into several domains. The N‐terminal part is composed of an intrinsically disordered region (IDR) followed by a structured domain called the N‐terminal domain (NTD). In this study, the structure determination of the N‐terminal region of the MERS‐CoV N protein via X‐ray diffraction measurements is reported at a resolution of 2.4 Å. Since the first 30 amino acids were not resolved by X‐ray diffraction, the structural study was completed by a SAXS experiment to propose a structural model including the IDR. This model presents the N‐terminal region of the MERS‐CoV as a monomer that displays structural features in common with other coronavirus NTDs.</div><div type="abstract" xml:lang="en">The structural characterization of the N‐terminal part of the nucleocapsid from Middle East respiratory syndrome coronavirus (MERS‐CoV), a recently emerging virus, is reported. The structure of the N‐terminal region, which includes a disordered tail followed by a globular domain, was obtained by combining X‐ray diffraction and SAXS.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><orgName><li>Université d'Aix-Marseille</li></orgName></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Papageorgiou, Nicolas" sort="Papageorgiou, Nicolas" uniqKey="Papageorgiou N" first="Nicolas" last="Papageorgiou">Nicolas Papageorgiou</name></region><name sortKey="Baklouti, Amal" sort="Baklouti, Amal" uniqKey="Baklouti A" first="Amal" last="Baklouti">Amal Baklouti</name><name sortKey="Baklouti, Amal" sort="Baklouti, Amal" uniqKey="Baklouti A" first="Amal" last="Baklouti">Amal Baklouti</name><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><name sortKey="Lichiere, Julie" sort="Lichiere, Julie" uniqKey="Lichiere J" first="Julie" last="Lichière">Julie Lichière</name><name sortKey="Lichiere, Julie" sort="Lichiere, Julie" uniqKey="Lichiere J" first="Julie" last="Lichière">Julie Lichière</name><name sortKey="Papageorgiou, Nicolas" sort="Papageorgiou, Nicolas" uniqKey="Papageorgiou N" first="Nicolas" last="Papageorgiou">Nicolas Papageorgiou</name><name sortKey="Papageorgiou, Nicolas" sort="Papageorgiou, Nicolas" uniqKey="Papageorgiou N" first="Nicolas" last="Papageorgiou">Nicolas Papageorgiou</name><name sortKey="Sevajol, Marion" sort="Sevajol, Marion" uniqKey="Sevajol M" first="Marion" last="Sévajol">Marion Sévajol</name><name sortKey="Sevajol, Marion" sort="Sevajol, Marion" uniqKey="Sevajol M" first="Marion" last="Sévajol">Marion Sévajol</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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